The central line catheter tip culture from the same hospital day was negative.

Rhizobium. Rhizobium anhuiense is very clearly an outgroup dark blue with all the Rlc strains. Rhizobium is the most well known species of a group of bacteria that acts as the primary symbiotic fixer of nitrogen. Take 20gms of agar 10gms of mannitol 05gms of di-potassium hydrogen phosphate 02gm of magnesium sulphate 01gm of sodium chloride 3gms of powered calcium carbonate 1gm of yeast extract and 1000 ml of distilled water in an Erlenmeyer flask plug its mouth.

Keywords---- Rhizobium legumes nodules biofertilizer nitrogen. Would decrease the need for chemical fertilizers and reduce adverse environmental effects. Spesies Rhizobium membentuk asosiasi pengikat nitrogen endosimbiotik dengan akar kacang-kacangan dan Parasponia.

Description and Significance. It is a fast growing bacteria which is a rich source of nitrogen to the crop. Rhizobium is the well-known nitrogen-fixing root nodule bacterial symbiont of legume plants see Rhizobia.

Nitrogen-fixation by organisms provides about 65 of the the biospheres. Rhizobium organisms in the soil recognize and invade the root hairs of their specific plant host enter the plant tissues and form a root nodule. To ensure adequate plant growth it is important to inoculate legume seed at planting with the correct strain of rhizobium.

They grow easily on conventional media produce oxidase and catalase and can be identified by most commercially available systems. That is why rhizobium requires a plant host. We found that members of a genospecies generally had ANI of 96 or above with the representative strain.

These bacteria can infect the roots of leguminous plants leading to the formation of lumps or nodules where the. Increasing and extending the role of biofertilizers such as. Bakteri ini mengkolonisasi sel-sel tumbuhan di dalam nodul akar di mana mereka mengubah nitrogen atmosfer menjadi amonia dan kemudian memberikan senyawa nitrogen organik seperti glutamina.