Sigma-Aldrich offers a number of Triton X-100 products.
Triton x-100. It is useful for isolation of membrane proteins. 112 A and B. It is also used in electrophoresis.
However if large amounts are added or the cells are. Coli Fjellbirkeland et al 1997. Triton X-100 is one of the most widely used non-ionic surfactants for lysing bacterial cells to extract protein and other cellular organelles or to permeabilize the living cell membrane.
The Triton X-100-based separation of the M. Unable to complete action likely due to connectivity to Dows back end service. Sigma assays dissolves 1 mL X-100 in 10 mL water to give a clear to slightly.
It is an active ingredient in influenza vaccine Fluzone. Schnaitman 1971abVisual inspection of whole cells and total membrane fractions with electron microscopy revealed structures reminiscent of intracytoplasmic membranes Fig. Detergent equivalent to Nonidet P-40Tritonr X-100 is used as a non-ionic detergent emulsifier and dispersing agent.
Triton X-100 a typical non-ionic detergent derives from polyoxyethylene and contains an alkylphenyl hydrophobic group. General description Triton X-100 has hemolytic property and is used for DNA extraction. Triton X-100 is a detergent commonly used in the biotechnology sector for laboratory activities such as cell lysis to extract proteins or to increase permeability of cell membranes.
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. By mixing 1 mL of Triton X-100 first with 10 mL of solution by pipetting up and down many times or shaking vigorously then adding this to the bulk solution and mixing more gently to avoid foaming. It is likely that many biotechnology start-ups use this detergent and have incorporated it.