C Yeast integrative plasmid.

Example of yeast expression vectors are. Detailed information is in Tschumper and Carbon 1980 Gene 10 157 Hartley and Donelson. The expression vectors are vectors which act as vehicles for DNA insert and also allow the DNA insert to be expressed efficiently. The expression vectors are genetically engineered for the introduction of genes into the target cells.

Yeast expression vectors such as YACs YIPs yeast integrating plasmids and YEPs yeast episomal plasmids have advantageous over bacterial artificial chromosomes BACs. YEps are vectors derived from yeast plasmids. METHODS IN ENZYMOLOGY VOL.

The expression vectors are also known as expression constructs. Some YEps contain the entire 2 micro-meter plasmids others just include the origin of replication. A Yeast episomal plasmid.

153 EXPRESSION AND SECRETION VECTORS FOR YEAST 517 Vectors designed for efficient expression of heterologous genes have been developed which employ promoter elements derived from the alcohol dehydrogenase 1 phosphoglycerate kinase 2 acid phosphatase 3 gly- ceraldehyde-3-phosphate dehydrogenase 4 galactokinase 5 and mating. These may be plasmids or viruses. Examples of yeast expression vector in Pichia are the pPIC series of vectors and these vectors use the AOX1 promoter which is inducible with methanol.

From novel vectors for bacterial mammalian or yeast protein expression to our complete kits for inducible tagged expression Agilent brings you a solution for every situation. The scheme for the construction of yeast expression plasmid designated pMA103 is. Some of the most widely used plasmids are pBR322 pUC and pBluescript vectors that use E.

An example is YRp7. Gives higher expression levels. Rapid growth in chemically defined media9product processing.