It is also known as hexa histidine-tag 6xHis-tag His6 tag by the US trademarked name HIS TAG and most commonly as His-Tag.

Example of expression vector with his tag. PSELECT-Tag is a family of expression plasmids designed to generate tagged proteins in order to facilitate their detection andor purification. Unlike other PrecisionShuttle vectors this vector does not have an Sgf I site at the multiple cloning site. Showing 7 of 7 suppliers 102933 products total Select All.

A derivative of this vector pAB-bee-FH encodes strong signal sequence and C-terminal dual FLAG-8xHis tag. PSELECT-Tag features three well-known tags. Your search returned 102933 His tag Conjugated Vectors Vectors across 7 suppliers.

Expression vectors 1. This vector is for Ecoli expression. A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine residues often at the N- or C-terminus of the protein.

In addition anti-His-tag antibodies are commercially available for use in assay methods involving His-tagged proteins. Horn V et al for example expressed RNF168-RING domain constructs in the petNKI-His-SUMO2-kan vector and purified using a His-SUMO tag and cleaved the tag using His-tagged SENP2 protease. The tag was invented by Roche although the use of histidines and its vectors are distributed by Qiagen.

Takes advantage of the HisTag fusion peptide also encoded by these vectors for HisBind metal chelation chromatography. You can use 5-CAT CAC CAT CAC CAT CAC-3 as a His tag sequence in any of N terminus or C terminus of gene sequnce. HisTag proteins can be purified under a wide range of either native or denaturing conditions.

PSELECT His-Tag plasmids allow to add the tag either at the N or C terminus of the protein of interest. Open product page in new tabwindow. For other reading frames use pcDNA31His B or pcDNA31His C.