The green fluorescent protein GFP gene the human influenza hemagglutinin HA epitope the polyhistidine His.

Example of expression vector with his tag. In either case the tag. MY QUESTION IS HOW DO I DESIGN PRIMERS WITH His-tag for the pKOV96 vector. PSELECT-Tag is a family of expression plasmids designed to generate tagged proteins in order to facilitate their detection andor purification.

Mammalian vector for expressing proteins with a cleavable N-terminal 6xHis tag. Select up to 5 products from below to compare or request more information. This tag could be histidine His tag or any other marker peptide.

A similar pAB-bee vector encodes a strong signal sequence and an 8xHis tag which is provided on the C- terminus of the translocated proteins but it does not encode for the N-terminal tag. Up to 20mg of fusion protein can be bound on a. The result is expression of a recombinant protein with a 6xHis or poly-His-tag fused to its N- or C-terminus.

C-terminal 6xHis tag ensures that only full-length protein is purified. Showing 7 of 7 suppliers 102933 products total Select All. Ickey TH et al expressed RIG-1 protein with a pET SUMO expression vector with an additional serine residue between the SUMO tag and RIG-1 to improve tag cleavage.

Unlike other PrecisionShuttle vectors this vector does not have an Sgf I site at the multiple cloning site. The HisBind affinity resin has a very high capacity for target proteins. Request Info for all products Compare.

Six consecutive Histidine residues 12. Empty lactoseIPTG inducible vector for high expression of a recombinant protein with a 6x His tag. I need to have 2 set of primers pET28 and pKOV96.